Studies on the substance which cause double .BETA. fraction on serum protein cellulose-acetate membrane electrophoresis.
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منابع مشابه
Estimation of serum proteins by electrophoresis on cellulose acetate.
We have evaluated results for albumin obtained by a standard procedure for cellulose acetate electrophoresis of proteins in serum. The Ponceau S-stained .albumin and globulins were eluted and the albumin was calculated by the generally accepted formula [(albumin-bound dye absorbance/absorbance of total protein-bound dye) X total serum protein concn] and by the formula (absorbance of albumin-bou...
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A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and y-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has aff...
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Our study of 95 serum samples from 37 patients with monoclonal gammopathy revealed distorted irregular monoclonal (M) protein bands after serum protein electrophoresis (SPE) on cellulose acetate membrane. In 71 (75%) of the 95 sera, the M-protein was underestimated and the albumin concentration overestimated. Dilution of the serum sample before SPE eliminated the abnormality of the M-protein ba...
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We studied the precision of densitometric quantification of the protein zones resolved by cellulose acetate electrophoresis. Replicate analyses of patients' samples by a single technologist showed mean CVs ranging from 2.9% for serum albumin to 9.5% for alpha 1-globulin. There were marked differences in measurements obtained by replicate analysis of the same samples by two experienced technolog...
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In previous papers (Gavrilescu, Jellinek, and McMenemey, 1961, Gavrilescu and McMenemey, 1964) on comparative studies on the protein fractions in the serum and cerebrospinal fluid in multiple sclerosis we have noted increases in the °C2 and f globulins in the serum. Although not specific for this disease these changes are of interest, particularly as multiple sclerosis is considered to be purel...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1994
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.38.7